Effects of Heat Needle Treatment on Inflammatory Reaction in Damaged Tissues of Rats with Third Lumbar Transverse Process Syndrome
10.13359/j.cnki.gzxbtcm.2017.05.018
- VernacularTitle:内热针治疗对第三腰椎横突综合征模型大鼠损伤组织炎症反应的影响
- Author:
Xiaojuan LUO
;
Zhaoqing ZHANG
;
Jing YIN
;
Yun XU
;
Yixuan DUAN
;
Yang XIA
;
Shiru JU
- Keywords:
heat needle;
third lumbar transverse process syndrome/acup-moxibustion therapy;
inflammatory reaction;
TNF-α;
IL-1β;
IL-6;
gene expression;
disease models,animal;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(5):703-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of heat needle treatment on the inflammatory reaction in the damaged tissues of rats with the third lumbar transverse process syndrome. Methods Ninety Sprague-Dawley rats were randomly divided into 6 groups, namely normal group, model group, drug group, heat needle group, conventional acupuncture group and warming acup-moxibustion group, 15 rats in each group. The rat model of the third lumbar syndrome was established by embedding gelatin sponge. Two weeks later, the rats in various groups were given corresponding treatment respectively. Before treatment and on the 7th, 14th and 28th day of treatment, the blood samples were collected from jugular vein, and then the serum inflammatory cytokines of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). On the 28th day of treatment, all of the rats were sacrificed, the expression levels of TNF-α, IL-1βand IL-6 mRNA in the muscle tissue were detected by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), and the pathological changes in local muscle tissue were observed by HE staining method. Results The serum contents of the inflammatory cytokines of TNF-α, IL-1β and IL-6 in the model group and treatment groups after modeling were significantly higher than those in the normal group (P < 0.05). After treatment, the contents of TNF-α, IL-1β and IL-6 in various treatment groups were decreased significantly as compared with those in the model group, and the decrease was most obvious in the heat needle group. The expression levels of TNF-α, IL-1β and IL-6 mRNA in muscle tissues of drug group, heat needle group, conventional acupuncture group and warming acup-moxibustion group were lower than those of the model group (P < 0.05), and the heat needle group had the lowest levels. HE staining results showed that heat needle group had milder inflammatory reaction and better muscle fiber structure than the model group. Conclusion Heat needle treatment can improve the local inflammatory response and promote the repair of tissue damage through decreasing the expression levels of inflammatory cytokines of TNF-α, IL-1βand IL-6.
