Establishment and Application of the Method of Polymorphism of Aldehyde Dehydrogenase 2 Gene Based on PCR-gold Magnetic Nanoparticle Chromatography

VernacularTitle:基于PCR-金磁纳米微粒层析技术检测乙醛脱氢酶2基因多态性方法的建立及应用
Author: Xiaoyue Lü ; Yuquan LIU ; Wenli HUI
Keywords: ALDH2; buccal swabs; goldmag nanoparticles chromatographic detection techniques
From: Journal of Modern Laboratory Medicine 2017;32(4):21-24
CountryChina
Language:Chinese
Abstract: Objective To estabolish the ALDH2 Glu504Lys genotyping technique for oral swab sample type based on PCR-gold magnetic nanoparticle chromatographyl.Methods According to the ALDH2 Glu504Lys gene the specific primers were designed by ARMS-PCR technique.Optimized the optimal ALDH2 Glu504Lys oral swab reaction system and PCR reaction procedures based on the routine reaction conditions and reaction procedure of laboratory PCR.60 samples of oral swabs were collected and tested by gold magnetic nano-chromatography.The results were verified by sequencing.Results The detected 60 cases of oral swab samples contain 16 cases heterozygous mutation,wild type 42 cases,homozygous mutation 2 cases and then the results by Goldmag nanoparticle detection was perfectly matched with sequencing results.Conclusion The Goldmag nanoparticles chromatographic detection technique of ALDH2 Glu504Lys buccal swabs system was established.With accuracy,fastness,reliability,it is worthy of clinical promotion.