Study on the Expression of Secretory Phospholipase A2-Ⅱ A in Lesions of Psoriasis Vulgaris
10.3969/j.issn.1671-7414.2017.04.026
- VernacularTitle:分泌型磷脂酶A2-Ⅱ A在寻常型银屑病皮损中表达的研究
- Author:
Yang ZHAO
;
Aimin XU
;
Shenglin LIU
;
Gang TIAN
- Keywords:
secretory phospholipase A2-Ⅱ A;
psoriasis vulgaris;
reverse transcriptase polymerase chain reaction;
skin lesion
- From:
Journal of Modern Laboratory Medicine
2017;32(4):93-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the relationship between the expression of secretory Phospholipase A2-Ⅱ A(sPLA2-ⅡA) and the pathogenesis psoriasis vulgaris patients lesions.Methods Using Psoriasis Area Severity Index(PASI),50 psoriasis vulgaris were divided into three groups:mild (n =16),moderate (n =18) and severe group (n =16),and compared to the non-psoriasis control group (n=52).The serum level of sPLA2-ⅡA in psoriasis vulgaris patients and the normal control group were detected using ELISA methods.Using RT-PCR,mRNA expression levels of sPLA2-ⅡA and subtypes were detected.Using western blot,the expression levels of Akt and p-Akt were detected.Results The serum level of sPLA2-ⅡA in the psoriasis lesion's group was significantly higher than that in the healthy control (t=13.62,P＜0.01).The mRNA expression of sPLA2-ⅡA in the lesion of psoriasis was significantly higher than that in health control (t=113.41,P＜0.01).In the other subtypes,the mRNA expression of sPLA2 had no distinctive.Among the three groups (mild group,moderate group and severe group),the difference in serum level and mRNA expression of sPLA2-ⅡA were statistically significant (F =28.12,69.62,P＜0.01).There was statistical significance among three groups (t=3.14,5.14,6.38,all P＜0.01;t=4.77,10.42,10.58,all P＜0.01).The expression of Akt/p-Akt in the psoriasis lesion's group were higher than that in health control (t=17.79,19.04,P＜0.01).Conclusion The expression of sPLA2-ⅡA would be associated with order of severity in lesions of psoriasis vulgaris.The pathway was relate to expression of Akt and p-Akt.
