Methodology Comparison Study on Two Analysis Systems of Procalcitonin
10.3969/j.issn.1671-7414.2017.04.041
- VernacularTitle:两种降钙素原检测系统比较实验研究
- Author:
Chenyu WANG
;
Liping YANG
;
Lei CHEN
;
Jing YANG
;
Fugang LI
;
Xiaoqiang SHI
- Keywords:
procalcitonin;
fluorescence lateral flow immunoassay;
electrochemical luminescence
- From:
Journal of Modern Laboratory Medicine
2017;32(4):143-144,147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the comparability of the test results of two immunoassay systems based on the electrochemical luminescence and the fluorescence lateral flow immunoassay for serum procalcitonin (PCT).Methods Roche cobas system was used as the reference system,and fluorescence lateral flow immunoassay system of Shanghai Upper biotech company was used as evaluated system.A total of 141 clinical samples during November,2015 were detected by the two systems to obtain the correlation coefficient and the Kappa values at the two cutoff values(0.5,2.0 ng/ml).Results The two systems showed high correlation (Y=1.008X-0.032,r=0.995,P＜0.001) and low deviation (t=-0.230,P=0.819＞0.05) without statistic significance between two methods.Kappa values were 0.944,0.943 respectively at the two cutoff values (0.5,2.0 ng/ml).Conclusion The test results showed no significant difference between the two immunoassay systems,suggesting a consistency between them for clinical detection of PCT.All the observed indicators reached the clinical diagnostic requirements and the method of quantitative detection of PCT by fluorescence lateral flow immunoassya can be applied for the quick detection of clinical human PCT.
