Determination of Adenosine Based on Ru(bpy)2+3/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor
10.11895/j.issn.0253-3820.170216
- VernacularTitle:基于Ru(bpy)2+3/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测
- Author:
Xuemei FAN
;
Shumin WANG
;
Zhejian LI
;
Xueyan JIA
;
Xingwang ZHENG
- Keywords:
Electrogenerated chemiluminescence;
Adenosine aptamer;
Adenosine
- From:
Chinese Journal of Analytical Chemistry
2017;45(9):1353-1359
- CountryChina
- Language:Chinese
-
Abstract:
Based on the AuNPs/Nafion composite membrane technology and immobilization of amino adenosine aptamer using carboxyl carbon nanotubes on the surface of a glassy carbon electrode, a electrochemiluminescence sensor was preparated.The sensor was characterized by cyclic voltammetry and electrochemical luminescence.The result showed that the sensor had a good stability and reproducibility.Adenosine and adenosine aptamer could form G-tetrahedral structure, leading a decrease of ECL intensity.Under the optimum experimental conditions, the relative ECL intensity showed a good linear relationship to the negative logarithm of adenosine concentration in the range of 1.0×10-11-1.0×10-7 mol/L, the linear equations was ΔIECL=-890lgC-5050 with a detection limit of 5.0×10-12 mol/L.The RSD was 2.7% in 11 times measurement of adenosine (1.0×10-10 mol/L).The recovery was 97.1%-110.0% in the determination of real adenosine sample.
