Effects of magnesium isoglycyrrhizinate on the expressions of UGT1A, MRP2 protein and mRNA in L-02 cells damaged by triptolide
10.11958/20170616
- VernacularTitle:异甘草酸镁对雷公藤甲素损伤L-02细胞中UGT1A、MRP2蛋白及其mRNA表达的影响
- Author:
Jing ZHANG
;
Shengnan ZHU
;
Qinyou TAN
- Keywords:
tripterygium;
multidrug resistance-associated proteins;
magnesium isoglycyrrhizinate;
triptolide;
uridine diphosphate glucuronyltransferase 1A;
MRP2;
L-02 cell
- From:
Tianjin Medical Journal
2017;45(9):912-916
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of magnesium isoglycyrrhizinate on the expressions of UGT1A, MRP2 protein and mRNA of L-02 cells damaged by triptolide, and to investigate hepatoprotective mechanism of magnesium isoglycyrrhizinate in terms of drug metabolism. Methods L-02 cells were divided into 4 groups:normal group, triptolide group, magnesium isoglycyrrhizinate group and rifampicin group. Magnesium isoglycyrrhizinate group and rifampicin group were pretreated by magnesium isoglycyrrhizinate and rifampicin for 24 h and the remaining two groups added medium. Triptolide were added for 18 h except normal group. Cell survival rate was tested by MTT. The expression levels of UGT1A, MRP2 protein and mRNA were detected by Western blot assay and RT-PCR. Results Compared with triptolide group, cell survival rate was significantly higher in magnesium isoglycyrrhizinate group (P<0.05). Meanwhile, the expression levels of UGT1A, MRP2 protein and mRNA were significantly lower in triptolide group compared with those of control group (P<0.05). The expression levels of UGT1A, MRP2 protein and mRNA were significantly up-regulated in magnesium isoglycyrrhizinate pretreatment group than those of triptolide group (P<0.05). The UGT1A protein and mRNA expressions were significantly decreased in rifampicin pretreatment group than those of magnesium isoglycyrrhizinate group ( P<0.05), but there were no significant differences in MRP2 protein and mRNA expressions between the two groups. Conclusion Magnesium isoglycyrrhizinate shows protective effects on triptolide induced L-02 cell injury, which may be involved with the activation of UGT1A and MRP2.
