Effects of an electromagnetic field on the proliferation and osteogenic differentiation of bone mesenchymal stem cells
10.3760/cma.j.issn.0254-1424.2017.08.002
- VernacularTitle:1.0mT低频交变电磁场对大鼠骨髓间充质干细胞增殖及成骨分化的影响
- Author:
Xiangyu TANG
;
Tao FU
;
Yang LIU
;
Chaoxu LIU
- Keywords:
Electromagnetic fields;
Stem cells;
Proliferation;
Osteogenic differentiation;
Mineralization
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2017;39(8):566-571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of an electromagnetic field (EMF) on the viability,proliferation and osteogenic differentiation of rats' bone mesenchymal stem cells (rBMSC).Methods Sinusoidal 1mT EMFs of 10 Hz,30 Hz,50 Hz and 70 Hz were administered to rBMSC for 2 hours per day during 2 weeks of cultivation.The cell viability was analyzed using live/dead assays.The cells' DNA was quantified to evaluate cell proliferation.Von Kossa staining was performed to study the mineralization of the extracellular matrix.The enzyme linked immunosorbent assay (ELISA) and immunohistological staining were employed to observe the synthesis of osteocalcin (OC) and bone morphogenetic protein-2 (BMP-2).Results Cell viability decreased under exposure to the 50 Hz and 70 Hz fields.The proliferation of rBMSC was elevated significantly in the 10 Hz EMF-treated group.The expression OC and BMP-2 was elevated after two weeks of 50 Hz EMF stimulation.Moreover,the mineralization of the extracellular matrix was found to be enhanced after 50 Hz EMF exposure.Conclusion Exposure to a 1 mT EMF has different effects on the viability,proliferation and osteogenic differentiation of rBMSC depending on the frequency.The data provide practical guidance for applying electromagnetic fields in bone regenerative medicine.
