Investigation of the effects induced by 1 400 W on nasopharyngeal carcinoma cell and the mechanism

VernacularTitle:1400W对鼻咽癌CNE-2细胞株的影响及机制探讨
Author: Caixia ZHANG ; Yangyun LIU ; Wen JIANG ; Ping WU ; Hua ZHENG ; Hang CAO ; Qiong CHEN ; Tao FAN ; Rong HU
Keywords: Nitric oxide synthase/AI/ME; Nasopharyngeal neoplasms/DT/ME
From: Journal of Chinese Physician 2017;19(8):1161-1164
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect induced by specific inducible nitric oxide synthase (iNOS) inhibitor 1 400 W on nasopharyngeal carcinoma CNE-2 cells lines and its mechanism.Methods CNE-2 cells were treated by different concentrations of 1 400 W,diamminedichloroplatinum (DDP),and both chemicals.Cell counting kit-8 (CCK-8) was used to examine the viability of cells.Quantitative realtime polymerase chain reaction (qRT-PCR) was applied to detect the iNOS mRNA expression.Results The expression of iNOS mRNA was down-regulated by 1400W and was positively correlated with inhibitionrate of cell proliferation.1 400 W inhibits proliferation of CNE-2 cell in a concentration-dependent manner.The proliferation inhibition rate of CNE-2 cells treated by 1 400 W combined with DDP was not enhanced.Conclusions Specific inducible nitric oxide synthase inhibitor 1 400 W can exerts anti-tumor effect though inhibiting the expression of iNOS mRNA;The mechanism of chemosensitization induced by iNOS inhibitor on CNE-2 cells may be closely related level of down-regulation of iNOS expression.