Effects of different concentration of IL-17 on the expression of RANKL and OPG in human periodontal ligament fibroblasts
10.3969/j.issn.1671-8348.2017.23.003
- VernacularTitle:白细胞介素-17对人牙周膜成纤维细胞表达RANKL、OPG的影响
- Author:
Linjuan LI
;
Min LI
;
Juanmin PENG
;
Na KANG
- Keywords:
periodontal ligament;
fibroblasts;
IL-17;
RANK ligand;
osteoprotegerin
- From:
Chongqing Medicine
2017;46(23):3177-3179,3183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different concentration of interleukin(IL)-17 on the expression of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprogeterin (OPG) in human periodontal ligament fibroblasts (HPDLF) and explore the relationship between IL-17 and orthodontic root resorption.Methods HPDLF cell line was established through the tissue pieces culture method in vitro.HPDLF were stimulated by IL-17 with five different concentrations(0,5,10,20,40 ng/mL) for 24 h.The expression level of mRNA and protein of RANKL and OPG in HPDLF were detected by RT-PCR and ELISA,respectively.Results HPDLF expressed RANKL and OPG in 0 ng/mL group.The expression amount of mRNA and protein of RANKL in HPDLF was positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.The expression amount of mRNA and protein of OPG was negative correlation with the concentration of IL-17 in 5 to 20 ng/mL group.The ralative RANKL/OPG ratio of mRNA and protein were positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.Conclusion HPDLF expresses RANKL and OPG in the absence of IL-17.IL-17 enhances the expression of RANKL and inhibits the expression of OPG in HPDLF.
