A preliminary study of AS1411 mediated STAT3 antisense oligonucleotide targeting tumor cells
10.3969/j.issn.1672-8467.2017.04.005
- VernacularTitle:AS1411介导STAT3反义寡核苷酸靶向抗肿瘤的作用
- Author:
Baoxiu LIU
;
Jiansheng HUANG
;
Naishuo ZHU
- Keywords:
antisense oligonucleotide;
AS1411;
anti-tumor;
targeting
- From:
Fudan University Journal of Medical Sciences
2017;44(4):422-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of AS1411-mediated signal transduction and activator of transcription 3 (STAT3) antisense oligonucleotide (ASO) targeting tumor cells.Methods RNA was used as coupling molecules to link the targeting molecules AS1411 and effector molecules ASO.Prediction and analysis of the secondary structure of the pre-synthesis of chimeric molecules by RNA Structure software.Agarose gel electrophoresis was used to test the stability of chimeric molecules in serum and cell lysis solution.Using flow cytometry and confocal fluorescence microscope were used to estimate the internalization of AS1411-mediated STAT3 ASO.Inhibitory effect of ASO on the growth of tumor cells was detected by CCK-8 kit.RT-PCR and Western blot was used to measure the expression levels of tumor related genes.Results STAT3 ASOs mediated by AS1411 can enter tumor cells efficiently to inhibit the transcription and translation of C-myc,Cyclin D1,Bcl-xl and PD-L1 gene,and also can inhibit the growth of Du145 cells.Conclusions AS1411-mediated STAT3 ASO can enter tumor cells and act as anti-tumor medicine.
