Effects of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α
10.3969/j.issn.1671-8348.2017.23.006
- VernacularTitle:槲皮素对TNF-α诱导的乳鼠心肌成纤维细胞增殖及P38MAPK、HMGB1蛋白表达的影响
- Author:
Chun ZHANG
;
Jian FENG
;
Jiafu LI
- Keywords:
quercetin;
fibroblasts;
myocardiu;
ventricular remodeling;
high mobility group;
proteins;
mitogen-activated protein kinase
- From:
Chongqing Medicine
2017;46(23):3189-3191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α.Methods Purified cardiac fibroblasts were obtained by trypsin digestion and differential adherence method.The proliferation of cardiac fibroblasts was detected by MTT assay.The expression of P38MAPK and HMGB1 protein was detected by Western blot.Results Quercetin had no effect on the proliferation of cardiac fibroblasts in the basal state but inhibited the proliferation of cardiac fibroblasts induced by TNF-α,and the A value of each group was increased with the increase of quereetin concentration(P＜0.05).The exspression of P38MAPK、HMGB1 were decresed with the icrease of quercetin.Conclusion Quercetin may inhibit the proliferation of cardiac fibroblasts induced by TNF-α.The mechanism may be inhibit the expression of HMGB1 through P38MAPK signaling pathway.
