Biological functions of SPA-PEI conjugate as one key component for construction of a novel antibody-targeted DNA delivery system
10.3969/j.issn.1671-8348.2017.23.022
- VernacularTitle:新型抗体靶向性核酸转移系统关键组分SPA-PEI交联物的生物学功能研究
- Author:
Yun LIU
;
Xiaofen LIU
;
Yuanhui ZHAI
;
Qiang WU
;
Xiaobo LIU
- Keywords:
staphylococal protein A-polyethylenimine conjugate;
DNA delivery system;
IgG binding;
DNA complexation
- From:
Chongqing Medicine
2017;46(23):3238-3240,3244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological function of SPA-PEI conjugate(staphylococcal protein A-polyethyleneimine cross-linker),which is one key component for construction of a novel antibody-targeted DNA delivery system.Methods The binding capacity of SPA-PEI conjugate with multiple sources of IgG was determined by enzyme-linked immunosorbent assay and neutralization inhibition assay.The binding capacity of SPA-PEI conjugate with DNA fragment was determined by DNA gel retardation assay,and its DNA condensing ability was measured by Ethidium bromide exclusion assay.Results SPA-PEI conjugate could bind well to many species-derived IgGs.SPA-PEI conjugate had no significant effect on the binding properties of SPA.SPAPEI conjugate could neutralize negative charges of the plasmid DNA or DzTi.Its DNA condensing ability was nearly same to that of free PEI,which suggested a excellent DNA condensing ability of the SPA-PEI conjugate.Conclusion SPA-PEI cross-linkers prepared by this project group maintained the biological activity of SPA and PEI.SPA-PEI cross-linkers could be used for the construction of a novel antibody-targeted non-virus DNA delivery system.
