Effects of aldehyde-free fixation solution and aldehyde fixation solution on the nucleus DNA and cytoplasm staining
10.3969/j.issn.1671-8348.2017.23.023
- VernacularTitle:无醛固定液与醛类固定液对细胞核DNA及细胞质着色的影响
- Author:
Rongxiang HUANG
;
Lanlan YU
;
Chenyan ZHU
;
Liang MA
;
Kun NIE
;
Wenbo HE
- Keywords:
pathology;
quantitative;
image cytometry;
cells,immobilized;
DNA
- From:
Chongqing Medicine
2017;46(23):3241-3244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aldehyde-free fixation solution and aldehyde fixation solution on DNA and cytoplasm of nucleus.Methods The DNA quantification (IOD),discrete coefficient (CV),DNA index (DI) and nucleus area (area) were measured by DNA quantification system (ICM) in 8 different fixation solution;combined with cytological staining,the effects of different fixative solution on nuclear DNA and cytoplasmic staining were analyzed.Results Aldehyde fixation solution was better than aldehyde free fixation solution,the formaldehyde in fixtion solution has important influence on Feulgen-eosin staining.Conclusion Aldehyde fixation solution combined with cytological staining can obtained good dyeing effect,which provides the basis for the subsequent multi-technique joint diagnosis.
