Study on molecular mechanism of double hydrogen artemisinin inducing apoptosis of bile duct cancer cells
10.3969/j.issn.1673-4130.2017.16.003
- VernacularTitle:双氢青蒿素DHA诱导胆管癌细胞凋亡的分子机制研究
- Author:
Jianming ZHU
;
Zhijian SHAO
- Keywords:
bile duct cancer;
double hydrogen artemisinin;
Mcl-1;
cell apoptosis
- From:
International Journal of Laboratory Medicine
2017;38(16):2183-2184,2187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and discuss the effect of double hydrogen artesunate on Mcl-1 expression and its inducing effect on cancer cell apoptosis in the patients with cholangiocarcinoma.Methods Bile duct cancer cell lines QBC939 preserved in our hospital from June 2010 to December 2014 were randomly selected and divided into the control group and observation group for conducting experiments.The cells were cultured by using the conventional cultivation and double hydrogen artemisinin culture.Then the Mcl-1 expression and apoptosis of cancer cells were performed the statistical analysis and comparison.Results Statistical comparison showed that the expressions of MCL1-001 and-MCL1 201 at 12,24,48 h in the observation group were significantly higher than those in the control group,the comparison between groups were statistically significant (P<0.05).MCL1-002 expression had little difference between at 12 h and 24 h (P>0.05),but which at 48 h in the observation was significantly higher than that in the control group,the difference was statistically significant(P<0.05).And the mortality rate at 6,12,24,48,72 h in the observation group was significantly higher than that in the control group,the difference was statistically significant (P<0.05).Conclusion Double hydrogen artemisinin has obvious up-regulation effect on Mcl-1,moreover can effectively induces bile duct cancer cell apoptosis.
