Blocked MyD88 signaling prevents OB after tracheal transplantation in mice
10.3760/cma.j.issn.0254-1785.2017.07.001
- VernacularTitle:阻断髓样分化分子88信号通路预防小鼠气管移植术后闭塞性细支气管炎的研究
- Author:
Min YANG
;
Xue ZHANG
;
Gen CHEN
;
Zuochuan DING
;
Ping ZHOU
;
Sheng CHANG
- Keywords:
lung transplantation;
tracheal transplantation;
innate immune;
MyD88;
TLRs
- From:
Chinese Journal of Organ Transplantation
2017;38(7):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of MyD88 in heterotopic tracheal transplantation in mice and its relationship with histopathological changes.Methods The mouse model of hetemtopictracheal transplantation was used.The mice were divided into three groups:(1) tracheal isograft of C57BL/6 to C57BL/6 mice;(2) tracheal allograft of BALB/c to C57BL/6 mice;(3) tracheal graft of BALB/c to C57BL/6 mice with MyD88 inhibitor treatment.The tracheal grafts were collected at indicated time points.Histological sections were stained with hematoxylin and eosin.The pathological changes were observed and their semi-quantitative measurement was done with Image J software.Results (1) Pathological results showed that the structure of the trachea with MyD88 inhibitor treatment was clear and the loss of epithelial cells was significantly reduced as compared with the positive control group at the time of 7 and 14 days.(2) The results of semi-quantitative measurement showed that luminal occlusion rate of MyD88 inhibitor treatment group was significantly reduced as compared with the positive control group (P＜0.01).However,the loss of epithelial cells was not improved 7 days after transplantation.Both of lumen occlusion rate (P＜0.05) and epithelial cells loss (P＜0.01) in MyD88 inhibitor treatment group were significantly reduced.Conclusion Inhibition of MyD88 molecule could significantly alleviate pathological changes of the transplanted trachea.Both of luminal occlusion rate and loss of epithelial cells were significantly ameliorated.
