Expression of Nupr1 in Gastric Cancer and its Clinical Significance
10.3969/j.issn.1008-7125.2017.08.004
- VernacularTitle:胃癌组织中Nupr1的表达及其临床意义
- Author:
Meiyu DING
;
Chao QI
;
Xiangjun JIANG
- Keywords:
Stomach Neoplasms;
Disease Progression;
Nucleoproteins;
Real-Time Polymerase Chain Reaction;
Blotting;
Western;
Immunohistochemistry
- From:
Chinese Journal of Gastroenterology
2017;22(8):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Background:The morbidity and mortality of gastric cancer remain high worldwide,and it is urgent to explore new targets for the diagnosis and treatment of gastric cancer. Nuclear protein 1 (Nupr1)has a variety of biological functions, especially in the tumorigenesis and development of the malignancies. Aims:To investigate the expression and clinical significance of Nupr1 in gastric cancer. Methods:Quantitative RT-PCR was used to determine the mRNA expression of Nupr1 in 72 cases of gastric cancerous tissue and the paired paracancerous tissue. Western blotting and immunohisto-chemistry were employed to detect the protein expression and cellular localization of Nupr1. The correlation of Nupr1 with the clinicopathological characteristics of gastric cancer was analyzed. Results:Expressions of Nupr1 mRNA and protein in gastric cancer were both significantly higher than those in paracancerous tissue (P < 0. 05),and their expressions were coincident. Nupr1 protein was expressed mainly in cytoplasm,and the nuclear expression was reduced. Expression of Nupr1 mRNA in gastric cancer was significantly correlated with tumor size,differentiation,pathological type,Bormann's classification,depth of invasion,lymph node and distant metastases and TNM staging (P < 0. 05),and was not correlated with gender,age and site of tumor (P > 0. 05). Conclusions:Nupr1 is overexpressed and correlated with invasion, metastasis and progression of gastric cancer. It can be used as a novel biomarker for early diagnosis and prognosis evaluation,and as a potential target for treatment of gastric cancer.
