Pathogenic characteristics and molecular typing of Salmonella strains isolated from diarrhea patients in Wuxi City, Jiangsu Province, China
10.3969/j.issn.1002-2694.2017.04.017
- VernacularTitle:无锡市腹泻病人沙门菌的病原学特征及分子分型研究
- Author:
Dan SHA
;
Hong LI
;
Hongxia GUAN
;
Weihong FENG
;
Yong XIAO
- Keywords:
Salmonella;
antibiotic resistance;
PFGE;
Luminex 200
- From:
Chinese Journal of Zoonoses
2017;33(4):378-381
- CountryChina
- Language:Chinese
-
Abstract:
We analyzed the pathogenic characteristics of Salmonella strains isolated from diarrhea patients in Wuxi City,Jiangsu Province,China and compared the differences among pulse field gel electrophoresis (PFGE) patterns of main serotype strains,so as to provid scientific basis for disease control.After biochemical identification of the Salmonella strains isolated from infectious diarrhea patients in Wuxi in 2015,drug susceptibility test,serotyping and PFGE were applied to analyze these strains.Results showed that a total of 32 Salmonella strains were detected from 756 diarrhea specimens with a positive rate of 4.23 ％.The infection occurred more frequently between May and October and adults aged more than 60 years old affected mostly.There was no significant difference between genders in infected population.The drug susceptibility test indicated that the antibiotic resistance rate of these Salmonella strains to ampicillin (56.25 ％) was the highest,and to ciprofloxacin(6.25 ％)and Ceftazidime (6.25％) were the lowest.The 32 Salmonella strains belonged to 11 serotypes,and S.enteritidis(31.25％)and S.typhimurium(21.88％) were the predominant serotypes.PFGE showed that the pattern similarity of all S.enteritidis was more than 85 ％;PFGE patterns of S.typhimurium were different.In conclusion,the infection of Salmonella from diarrhea patients in Wuxi City had obvious season and age specific distribution,and the most prevalent serotype of Salmonella was the S.enteritidis.It is necessary to strengthen the surveillance of Salmonella concurrently in food and environment.
