Identification and analysis of arsenic interactors byEscherichia coli proteome microarray

VernacularTitle:大肠埃希菌蛋白质组芯片对砷相互作用蛋白的检测分析
Author: Yin LIU ; Lina YANG ; Hainan ZHANG ; Shengce TAO
Keywords: arsenic; proteome microarray; Escherichia coli; biological metabolism
From: Journal of Shanghai Jiaotong University(Medical Science) 2017;37(5):583-587
CountryChina
Language:Chinese
Abstract: Objective · To globally study the influence of arsenite to the various biological pathways of Escherichia coli as a model organism.Methods · The protein-arsenite interactions was globally studied based on a proteome microarray constructed by 4256 affinity-purified Escherichia coli proteins. The functions of interacting proteins and their network were then analyzed by bioinformatics. Results · 91 proteins that remarkably interact with arsenic were successfully identified. Bioinformatics analysis found that most of the proteins possess catalytic activityand are involved in various biosynthesis and cellular metabolism pathways. The interactions of arsenic with proteins encoded by malY, cfa and hypF genes were further validated by Western blotting, which proves the results of proteome microarray reliable. Conclusion · Arsenite interacts with a variety of enzymes ofEscherichia coli and can greatly affect its biological metabolism.