Isolation and characterization of bacteriophage against extended-spectrumβ-lactamase-producing Escherichia coli strains
10.3969/j.issn.1674-8115.2017.08.004
- VernacularTitle:产超广谱β-内酰胺酶大肠埃希菌噬菌体的分离及特性研究
- Author:
Leijie HUANG
;
Xiaoliang LIU
;
Rui ZHU
;
Fangqi LIN
;
Jinhong QIN
;
Ping HE
;
Xiaokui GUO
;
Wei ZHAO
- Keywords:
extended-spectrum β-lactamase-producing Escherichia coli;
phages;
biological characteristics;
genomic characteristics
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(8):1069-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective · To isolate phages which can fight against extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli (E. coli), and provide basic research for establishment of E. coli phage library and treatment of bacterial infection. Methods · Samples collected from sewage were co-cultured with 93 ESBLs-producing E. coli strains. A phage named JDEC001 was isolated by double agar overlay plaque assay. The biological characteristics, complete genome sequence and comparative genome analyses of JDEC001 were studied respectively. Results · JDEC001 belongs to the lytic phage as a member of the Caudovirales order, Podoviridae family. It has high activity at pH from 5 to 11 and with temperature from 0 to 39 ℃ .Whole-genome sequencing of JDEC001 demonstrated double-stranded DNA genome of 38745 bp with GC content of 49.93%, which encoded 46 open reading frames. The comparative genomics also showed that there was no virulent genes or antibiotic resistant genes in its genome. Conclusion · The phage JDEC001 against ESBLs-producing E. coli was isolated and purified, with good stability in a broad range of pH and temperature.
