Investigation on spoligotyping and phenotypes of drug resistance to four first-line drugs in 251 Mycobacterium tuberculosis isolates from Qinghai, China
10.3969/j.issn.1002-2694.2017.04.008
- VernacularTitle:青海省251株结核分枝杆菌Spoligotyping基因型与4种一线药物耐药表型的研究
- Author:
Zhaofen WANG
;
Bin LI
;
Mingxia JIANG
;
Yongcheng MA
;
Haijing WANG
;
Xiuli SHEN
;
Xueqiong YAO
;
Tingting LI
;
Ying CHEN
;
Xinhua WANG
- Keywords:
Mycobacterium tuberculosis;
drug resistance;
spoligotyping
- From:
Chinese Journal of Zoonoses
2017;33(4):332-336
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to learn drug resistance situation of four first-line anti TB drugs among 251 Mycobacterium tuberculosis isolates from Qinghai and to explore their relationships with genotypes by Spoligotyping,so as to provide basis for effective prevention of tuberculosis.Isolates of 251 M.tuberculosis were tested susceptibilities of four first-line drugs including isoniazid (INH),rifampicin (RFP),streptomycin(SM) and ethambutol (EMB) by using conventional proportion method and genotyped by Spoligotyping.Relationship between drug resistance and genotypes was analyzed statistically.Results showed the total drug resistance rate was 56.2％ (141/251) among 251 M.tuberculosis isolates.Resistance rates of four first-line drugs were 43.0％ (108/251) for INH,37.1％ (93/251) for RFP,39.0％ (98/251) for SM,27.9％ (70/251) for EMB respectively.Rate of multidrug-resistant TB (MDR TB) was 31.5％ (79/251).All 251 isolates of M.tuberculosis were typed by spoligotyping.The 185 (73.7 ％) were Beijing genotypes and 66 (26.3.％) were non-Beijing genotypes,and no statistical association was found with drug resistance.This paper concludes that isolates of M.tuberculosis prevail in Qinghai have both high rates of drug resistance and MDR and dominant isolates are Beijing genotypes by spoligotyping.
