Gender dependence of metabolomic phenotypes for human saliva using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry
10.3969/j.issn.1674-8115.2017.08.006
- VernacularTitle:超高效液相色谱-四极杆飞行时间质谱联用技术分析人类唾液代谢组的性别依赖性
- Author:
Jing LI
;
Si LIU
;
Hehua LEI
;
Yulan WANG
;
Huiru TANG
- Keywords:
saliva;
metabolomics;
ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry;
gender dependence
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(8):1079-1084
- CountryChina
- Language:Chinese
-
Abstract:
Objective · To investigate the gender-related metabolomic differences in human saliva. Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences.Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites,7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine,isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males. Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva.
