Establishment of a rat model of hyperuricemia associated with uric acid excretion disorder

VernacularTitle:尿酸排泄不良型高尿酸血症动物模型的建立
Author: Guiqin SHEN ; Shijia YU
Keywords: Hyperuricemia; Potassium oxonate; Pyrazinamide; Ethambutol; Rat model
From: Chinese Journal of Comparative Medicine 2017;27(8):55-59
CountryChina
Language:Chinese
Abstract: Objective To explore a reliable method to establish a rat model of hyperuricemia associated with abnormal uric acid excretion, and to lay the foundation for the study of pathogenesis of uric acid excretion disorder and the optimization of the treatment plan.Methods The models were established respectively by potasium oxonate(300 mg/kg) with pyrazinamide (300 mg/kg) or ethambutol(250 mg/kg).Continuous dosing for 1, 3 and 5 weeks, to determine the content of uric acid in rat blood, urine, and stool, the function of liver and kidney was detected and pathological examination was performed.Results The blood uric acid in the potasium oxonate and ethambutol group was increased first and then decreased, while in the potasium oxonate and pyrazinamide group were increased steadily and the excretion of uric acid in urine was stable during the continuous administration.The two methods showed no harmful effect on the liver and kidney function.Conclusions A stable rat model of hyperuricemia associated with uric acid excretion disorder can be effectively established by potassium oxonate and pyrazinamide, exhibiting similar manifestations of clinical hyperuricemia and uric acid excretion disorder.