Inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on immune activity of T cells in mice

Minying Sun; Guomu Liu; Jing Jie; Fei Xie; Ruiping Zhai; Tanxiu Chen; Hongyan Yuan; Guixiang Tai

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Inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on immune activity of T cells in mice

VernacularTitle:重组MUC1-MBP融合蛋白联合R848对小鼠T细胞免疫活性的诱导作用
Author: Minying SUN ; Guomu LIU ; Jing JIE ; Fei XIE ; Ruiping ZHAI ; Tanxiu CHEN ; Hongyan YUAN ; Guixiang TAI
Keywords: MUC1-MBP; R848; cancer vaccine; adjuvant
From: Journal of Jilin University(Medicine Edition) 2017;43(3):507-511
CountryChina
Language:Chinese
Abstract: Objective:To study the inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on the immune activity of T cells in the mice,and to provide experimental basis for searching the suitable adjuvants of recombinant MUC1-MBP fusion protein.Methods:A total of 21 C57BL/6 mice were randomly divided into control group(treat with normal saline),MUC1-MBP+R848 group (treated with MUC1-MBP+R848),and MUC1-MBP+baillus calmette guerin(BCG) group(treated with MUC1-MBP+BCG)(n=7).After immunized for 4-7 d,the spleen tissue was taken and the spleen indexes of the mice in various groups were measured;the stimmulus index(SI) of the mice was detected by lymphocyte proliferation response;the levels of tumor necrosis factor-γ(TNF-γ) and interleukin-4(IL-4) were detected by ELISA method;the proprotions of T lymphocyte subsets in spleen cells were analyzed by flow cytometry.Results:Compared with control group,the spleen indexes,SI,and TNF-γ levels of the mice in MUC1-MBP+ R848 and MUC1-MBP+BCG groups were significantly increased (P<0.05 or P<0.01);the indexes metioned above of the mice in MUC1-MBP+ R848 were higher than those in MUC1-MBP+BCG group;the IL-4 levels had no significant difference(P>0.05).Compared with control group,the proprotions of CD3+T,CD4+T,and CD8+ T cells of the mice in MUC1-MBP+ R848 group and MUC1-MBP+BCG group were significantly increased(P<0.05 or P<0.01).Conclusion:Both MUC1-MBP fusion protein combined with R848 and BCG can induce the Th1 type of immune activity in the mice,and R848 is the potential candidate adjuvant for MUC1-MBP.