A tentative exploration of the expression of B-1a cells in mice with obesity and periodontal infection
10.3969/j.issn.1001-3733.2017.03.001
- VernacularTitle:肥胖伴牙周感染小鼠体内B-1a细胞的表达初探
- Author:
Yixiong WANG
;
Ting YU
;
Baoyi XIE
;
Dongying XUAN
;
Jincai ZHANG
- Keywords:
Periodontitis;
Obesity;
Jaw bone;
Spleen;
B-1a cells
- From:
Journal of Practical Stomatology
2017;33(3):287-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of B-1a cells in mice with obesity and periodontal infection.Methods:Mouse models of diet induced obesity combined with experimental periodontitis were established,the expression of CD5 protein,anti-collagenⅠ antibody(anti-Col-Ⅰantibody) and IL-10 protein was examined in mouse jaw bone and spleen by immunohistochemistry and Western blot;The mRNA expression of CD5,anti-Col-Ⅰantibody and IL-10 in mouse jaw bone was detected by real time quantitative PCR.Results:The mRNA and protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in jaw bone in periodontitis group were significantly higher than those in control group(P<0.001).The protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in spleen in obesity group were significantly higher than those in standard group(P<0.05).The protein expression of anti-Col-Ⅰantibody in spleen in standard accompanying periodontal ligature group was significantly higher than that in standard without periodontal ligature group(P<0.05).Conclusion:B-1a cells are activated in the early stage of obesity and periodontal inflammation with a certain pathological significance and without interation between the two inflammatory states in the pathological mechanism.
