Application value of CMIA in HCV infection validation in low risk population
10.3969/j.issn.1673-4130.2017.10.003
- VernacularTitle:化学发光微粒子免疫测定技术对低风险人群HCV感染确证的应用价值
- Author:
Linfeng WU
;
Jinfeng ZENG
;
Yuanzhang SUN
;
Jieping LONG
;
Lilin WANG
- Keywords:
chemiluminescence microparticle immunoassay;
recombinant immunoblotting assay;
immunosorbent assay;
hepatitis C virus;
confirmation of infection
- From:
International Journal of Laboratory Medicine
2017;38(10):1301-1303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the reactive samples of enzyme-linked immunosorbent assay (ELISA1) by chemiluminescence microparticle immunoassay (CMIA),and to analyze the application value of CMIA in HCV infection validation of blood donors.Methods Nucleic acid 3-item combined testing (NAT),another ELISA2,HCV antibody supplementary test(Western Blot test,WB) and CMIA test supplemented in blood samples of 102 ELISA1 anti-HCV reactive blood donors were retrospectively analysed.Results Among 102 blood donors of anti-HCV positive,32 cases (31.37%,32/102) were HCV RNA reactive samples,50 cases (49.02%,50/102) were ELISA2/WB reactive simultaneously.With CMIA NAT results as the reference standard,CMIA was poorly correlated with HCV RNA (Spearman correlation coefficient rs=0.395,P<0.01),and the consistency between them was weak by Kappa test (Kappa=0.270,P<0.01).With ELISA2/WB detection results as the reference standard,CMIA was highly correlated with the results(Spearman correlation coefficient rs=0.713,P<0.01),and which showed high consistency by Kappa test (Kappa=0.674,P<0.01).Conclusion CMIA as a detection method of protein label after HCV infection has great value in the HCV infection confirmation in low-risk population.