Influence of RNAi inhibiting JMJD3 gene expression on growth,proliferation and invasion of hepatocellular carcinoma cells
10.3969/j.issn.1673-4130.2017.10.016
- VernacularTitle:RNA干扰抑制JMJD3基因表达对肝癌细胞生长,增殖和侵袭的影响
- Author:
Peipei ZHANG
- Keywords:
RNA interference;
JMJD3;
hepatocellular carcinoma
- From:
International Journal of Laboratory Medicine
2017;38(10):1333-1335
- CountryChina
- Language:Chinese
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Abstract:
Objective To study the influence of small interfering RNA(siRNA) inhibiting Jumonji-containing JMJD3 gene on growth,proliferation and invasion of hepatocellular carcinoma cells.Methods JMJD3 was silenced by RNA interference(siR-JMJD3),non-specificity sequence pSilercer 2.1 was transfected into liver cancer cells QGY 7703 and HepG2 cells as the negative control.MTT assay,colony formation and Transwell invasion assays were used to detect the cell growth,proliferation and invasion ability of hepatocellular carcinoma cells.Results The Western Blot detection results showed that the siR-JMJD3 plasmid transfected experiment group successfully inhibited the JMJD3 protein expression level in liver cancer cells.The MTT results showed that after transfecting siR-JMJD3,the growth activity of QGY-7703 and HepG2 were decreased by 25% or 17% compared with the control group,.The colony formation results showed that in the colony formation number of two cell lines were decreased by 31% and 25% respectively.The Transwell invasion test results indicated that transmembrane cells number was decreased by 44% and 47% respectively.Conclusion Using siRNA technique can effectively inhibit JMJD3 gene expression,meanwhile effectively inhibits the in vitro growth,proliferation and invasion of liver cancer cells QGY-7703 and HepG2 and provides a new thinking for the biological therapy of liver cancer.
