Analysis on interference effects of vitamin C on three kinds of glucose detection methods
10.3969/j.issn.1673-4130.2017.10.017
- VernacularTitle:维生素C对3种血糖检测方法的干扰效应分析
- Author:
Jing ZHOU
;
Wei YAN
;
Jianfeng SHI
;
Ye JIANG
;
Tingting CAI
;
Xiaoming YAO
- Keywords:
vitamin C;
blood glucose;
interference;
hexokinase method;
glucose oxidase method
- From:
International Journal of Laboratory Medicine
2017;38(10):1336-1337,1340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interference effects of vitamin C on three different detection methods of blood glucose.Methods According to CLSI EP7-A2 document,5% volume of vitamin C(series concentrations) solution was added to fresh mixed serum,then hexokinase method(A),glucose oxidase method(B) and glucose reductase electrode method(C) were used to detect the level of fresh mixed serum glucose.The interference level of vitamin C on three different detection methods of blood glucose was evaluated.Results In 1 250 mg/L vitamin C,there was no interference on the method A for detecting blood glucose.In 156 mg/L vitamin C,it caused unacceptable negative interference on the method B and unacceptable positive interference on the method C.Moreover the interference degree of these two methods was increased with vitamin C concentration increase,but the same concentration of vitamin C had no significant difference on detecting different blood glucose levels by the same detection method.Conclusion High concentration of vitamin C can cause significant interference on glucose detection by glucose oxidase method and glucose reductase electrode method.
