A new model of rat artery transplantation
10.3969.j.issn.1671-7856.2017.05.021
- VernacularTitle:一种新型大鼠动脉移植模型的建立
- Author:
Lailong SUN
;
Jianping DU
;
Chaobing LIU
;
Songlin ZHANG
- Keywords:
Allograft vasculopathy;
Transplantation;
Model
- From:
Chinese Journal of Comparative Medicine
2017;27(5):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective Allograft Vasculopathy(AV), which limits the function and long-term survival of transplanted organs, it is the most urgent problem need to be solved in the field of organ transplantation.Traditional vascular anastomosis requires excellent microsurgical techniques, it is difficult to carry out.To explore a simple and feasible AV model can provide ideas for clinical research.Methods We make vessel cannulas by using vein detained needles, pulling the thoracic aorta of donor rat in vessel cannula, both ends turned over and fixed,and then transplant it in recipient rat abdominal aorta.1, 4 and 8 weeks after transplantation, we take out of the transplanted arteries, HE stain, to observe the histomorphology and measurement the vascular intimal thickness.Results 38 of the 40 recipient rats healthy survival to the detection time points, the legs and tail can move freely, defecating and urinating normally.It is found that transplanted arteries concentric circles thickening, its histomorphology just like the AV by using HE staining.Conclutions The method of rats artery transplantation is simple and feasible, significantly shorten the time of abdominal aortic blocking, reduce surgical trauma, can improve the success rate and the reproducibility of operation.
