Origin Determination of Sika Deer Bones by Restriction Fragment Length Polymorphism
- VernacularTitle:利用限制性片段长度多态性技术鉴别梅花鹿骨种属来源的研究
- Author:
Ziqiang WANG
;
Jing Lü
;
Hong SHAO
;
Ruixue XIA
;
Gang CHEN
- Keywords:
Bones of sika deer;
Restriction fragment length polymorphism;
PCR;
Species identification
- From:
China Pharmacist
2017;20(5):813-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method of restriction fragment length polymorphism (RFLP) to determine the origin of sika deer bones.Methods: The DNA in the bone samples was extracted after decalcification, and then amplified using polymerase chain reaction (PCR).The origin of the samples was further identified using RFLP analysis.Results: The bone samples of sika deer and red deer could be distinguished from those of pig, bovine and dog by PCR.And the samples of sika deer and red deer could be further distinguished by RFLP through the analysis of the length of restriction enzyme XbaI.Conclusion: A RFLP method is established to determine the origin of sika deer bones.
