The effects of experimental periodontitis on serum C reactive protein and insulin resistance in obese rats
10.3969/j.issn.1001-3733.2017.03.009
- VernacularTitle:实验性牙周炎对肥胖大鼠血清C反应蛋白及胰岛素抵抗的影响
- Author:
Sulan ZHONG
;
Jia NI
;
Qiaoxue CHAI
;
Jincai ZHANG
;
Lei CHEN
- Keywords:
Experimental periodontitis;
Obesity;
Insulin resistance;
C-reactive protein(CRP)
- From:
Journal of Practical Stomatology
2017;33(3):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of experimental periodontitis on serum C reactive protein(CRP) and insulin resistance in obese rats.Methods:4 week old SD rats (n=35)were fed with high fat diet for 16 weeks to establish the model of obesity.According to the obesity criteria,5 rats were screened out.According to the proportion of 1:2,10 rats were randomly selected as obese control group,20 obese rats were used for the establishment of the obesity with periodontitis model(combination group) by periodontal ligation using silk thread.4 rats died after ligation.Before periodontal ligation,1 and 4 weeks after periodontal ligation,orbital venous blood were collected for the detection of fasting blood glucose and fasting insulin.Homeostasis model assessments of insulin resistance index(HOMA-IR) and beta cell function index(HOMA-β) were calculated.The level of serum CRP was examined by ELISA.Results:The level of CRP in the combination group was increased 1 week after periodontal ligation,and then gradually decreased in the following 4 weeks(F=7.773,P=0.004).HOMA-IR in the combination group were higher than that in the obese group(F=-4.691,P=0.000),and beta cell function index was significantly lower(F=3.672,P=0.002) than that in the control group 4 weeks after periodontal ligation.Conclusion:The experimental periodontitis may affect the serum level of CRP in obese rats,aggravate insulin resistance and decrease the function of beta cells in obese rats.
