The effects of human dental pulp stem cells-conditioned medium on the osteogenic differentiation of human dental follicle stem cells in vitro

VernacularTitle:人牙髓细胞条件培养液对人牙囊细胞成骨分化作用的体外研究
Author: Yu TAO ; Jinfeng YU ; Jun CHEN ; Yong YU
Keywords: Human dental follicle stem cells(HDFSCs); Human dental pulp stem cells-conditioned medium(HDPSCs-CM); Microenvironment; Osteogenic differentiation
From: Journal of Practical Stomatology 2017;33(3):339-343
CountryChina
Language:Chinese
Abstract: Objective:To evaluate the effects of human dental pulp stem cells-conditioned medium(HDPSCs-CM)on the osteogenic differentiation of human dental follicle stem cells(HDFSCs) in vitro.Methods:HDFSCs were in vitro cultured,purified and identified.CCK-8 assay was applied to evaluate the HDFSCs viability after 7 days cultured by HDPSCs-CM;the morphological changes of HDFSCs were observed;the osteogenic differentiation was studied by alkaline phosphatase(ALP) staining and alizarin red staining.The mRNA expression of POSTN,Col-Ⅰ,ALP,BSP and OPN was detected by Real-Time PCR.Results:Induced by HDPSCs-CM,HDFSCs exhibited several characteristics of cementoblast or osteoblast lineages.In the induction group the viability of HDFSCs was inhibited(P<0.05 or P<0.01),ALP staining was stronger and there were more mineralized nodules,the expression levels of POSTN,Col-Ⅰ,ALP,BSP and OPN were upregulated(P<0.05 or P<0.01).Conclusion:HDPSCs-CM can promote the osteogenic differentiation of HDFSCs.