The influence of thyroid hormones on the expression of NGF and BDNF after cerebral ischemia-reperfusion injury in rats
10.3969/j.issn.1671-8348.2017.15.005
- VernacularTitle:甲状腺激素T3对大鼠脑缺血再灌注损伤后NGF和BDNF表达的影响研究
- Author:
Chao DOU
;
Min ZHANG
;
Yuanzheng ZHAO
;
Yapei GUO
;
Shitao WU
;
Hengfang LIU
- Keywords:
brain ischemia;
reperfusion injury;
thyroid hormones;
nerve growth factor;
neuroprotective effect;
brain-derived neurotrophic factor
- From:
Chongqing Medicine
2017;46(15):2030-2033
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effect of thyroid hormones T3 on cerebral ischemia-reperfusion injury in rats and its mechanism.Methods SD rats were divided into four groups:sham+saline group,sham+T3 group,MCAO+saline group,MCAO+T3 group.The cerebral ischemia-reperfusion injury rat models were established by right middle cerebral artery occlusion.Thyroid hormones(10 μg/100 g)or normal saline were given respectively by intraperitoneal injection twice at 1 h after the onset of ischemia and 6 h after reperfusion.Neurobehavioral score was evaluated at 24 h after reperfusion;TTC staining was used to label infarction area;RT-PCR was used to detect the mRNA level of nerve growth factor(NGF)and brain derived neurotrophic factor(BDNF)in brain tissue;Western blot was employed to determine alterations in protein levels of NGF and BDNF.Results Compared with MCAO+saline group,the neurological deficit and the volume of cerebral infarction of MCAO+T3 group was decreased,and the mRNA and protein expression of NGF and BDNF of MCAO+T3 group were increased(P<0.05).Conclusion Thyroid Hormones could promote the nerve repair,stimulate the nerve regeneration and improve the nervous behavioral function by up-regulating the expression of NGF and BDNF.
