Effects of different titers of bacteriophage D29 on growth and function of airway epithelia cell 9HTE
10.3969/j.issn.1671-8348.2017.15.003
- VernacularTitle:不同滴度噬菌体D29对气道上皮细胞9HTE的生长及功能影响研究
- Author:
Mei LIANG
;
Shuliang GUO
;
Hongmei ZHANG
- Keywords:
bacteriophages;
trachea;
interleukins;
vascular cell adhesion molecule-1;
airway epithelial cells;
security
- From:
Chongqing Medicine
2017;46(15):2024-2026
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the effects of different titers of bacteriophage D29 on growth and function of airway epithelial cell 9HTE.Methods Cell viability rates was analyzed after applying high(109 PFU/mL)and low(107 PFU/mL)titers of bacteriophage D29 and phage buffer respectively by MTT colorimetry.Additionally,the secretion levels of IL-6,IL-8 in cell culture supernatant were detected by ELISA.RT-PCR was performed to detect the expression of ICAM-1 mRNA.Cell apoptosis rate was analyzed by flow cytometry.Results There was no difference in cell growth,secretion levels of IL-6,IL-8,ICAM-1 mRNA and cell apoptosis rate between cells treated with high and low titers of D29 and phage buffer(P>0.05).Conclusion Neither high nor low titer of bacteriophage D29 exerts effect on growth and function of airway epithelial cell 9HTE in vitro.
