Significance of K-ras gene status and ras protein expression in immunophenotypic classification of gastric signet ring cell carcinoma
10.3969/j.issn.1671-8348.2017.15.004
- VernacularTitle:K-ras基因状态及ras蛋白表达在胃印戒细胞癌免疫表型分类中的意义
- Author:
Qiuying XIONG
;
Xin XIONG
;
Xiuhua HU
;
Luxia TU
;
Zhenfang XIONG
- Keywords:
stomach neoplasms;
genes;
ras;
proteins;
signet ring cell carcinoma;
K-ras gene;
lymph node metastasis;
targeting therapy
- From:
Chongqing Medicine
2017;46(15):2027-2029,2033
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of K-ras gene status and ras protein expression in immunophenotypic classification of gastric signet ring cell carcinoma.Methods The expression of ras protein in 180 cases of gastric signet-ring cell carcinoma was detected by tissue microarray immunohistochemistry.Meanwhile,the mutation in codon 12,13 of K-ras gene was determined by using PCR-based DNA direct sequencing analysis.Results The rate of ras protein expression was 27.8％.The rate of ras protein expression in intestinal phenotype was significantly higher than those in gastric and gastrointestinal phenotypes(P<0.05).The rate of ras protein expression in cases with lymph node metastasis was significantly higher than those in cases without nodal involvement(P<0.05).The rate of ras protein expression was significantly higher in cases with deeper invasion(P<0.05).The frequency of K-ras gene mutation was 22(12.2％).All of them were found in codon 12.The types of mutation included GGT→AGT(1 case),GGT→TGT(1 case),GGT→GCT(2 cases),GGT→GTT(8 cases)and GGT→GAT(10 cases).K-ras mutation was significantly associated with intestinal phenotype(P<0.05).The rates of ras protein expression in cases with mutational type of K-ras gene was higher than those in cases with wild type(P<0.05).The ras protein expression was positively associated with K-ras gene mutation(r=0.61,P<0.05).Conclusion The ras protein expression is correlated with nodal involvement and invasion.K-ras gene mutation and expression of ras protein is related to phenotypic classification,and they might influence the phenotypic transformation in gastric signet ring cell carcinoma.
