Effect of ghrelin on proliferation and mitofusin-2 expression of human aortic smooth muscle cells
10.3969/j.issn.1671-8348.2017.15.006
- VernacularTitle:胃促生长素对人主动脉平滑肌细胞增殖及线粒体融合蛋白2表达的影响
- Author:
Yongming HE
;
Mingbao SONG
;
Jianbo HU
;
Yuanping ZHANG
;
Youmei LI
- Keywords:
ghrelin;
muscle;
smooth;
vascular;
aorta;
human aortic smooth muscle cells;
proliferation;
mitofusin 2
- From:
Chongqing Medicine
2017;46(15):2034-2036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ghrelin on proliferation of vascular smooth muscle cells(VSMC)and the expression of mitochondrial fusion 2(Mfn-2)in cultured human aortic smooth muscle cells(HASMCs).Methods HASMCs were cultured in vitro,treated with different concentrations(10-9,10-8,10-7,10-6,10-5 mol/L)ghrelin or 10-6 mol/L ghrelin for different time(0,6,12,18,24 h).Subconfluent HASMCs at passage 4-6 were used in experiments.MTT essay was used to investigate the effect on proliferation of HASMCs.RT-PCR and Western blot were used to analyse the expression of Mfn-2.Results 10-7-10-5 mol/L ghrelin inhibited the proliferation of HASMCs,and the inhibitory effect of concentration of 10-6 mol/L was the most obvious(P<0.01).Ghrelin inhibited the proliferation of HASMCs in 6-24 h,and it reached the peak at 24 h(P<0.01).10-6 mol/L ghrelin significantly increased the expression of Mfn-2 mRNA and protein(P<0.01).The up-regulation of 10-6 mol/L ghrelin on Mfn-2 mRNA and protein expression reached the peak at 18 h(P<0.01).Conclusion Ghrelin might inhibit the proliferation of HASMC by up-regulating the expression of Mfn-2.
