Effect of miR-204 in cell biological characteristics of breast cancer MDA-MB-231 cell
10.3969/j.issn.1671-8348.2017.14.004
- VernacularTitle:miR-204对乳腺癌MDA-MB-231细胞生物学特性的影响
- Author:
Liucheng WU
;
Lili DU
;
Jing WANG
;
Hailin YIN
;
Chao MA
;
Maorong JIANG
;
Yixiang SHAO
- Keywords:
breast neoplasms;
apoptosis;
MicroRNAs;
miR-204;
MDA-MB-231;
cell biological characteristics
- From:
Chongqing Medicine
2017;46(14):1881-1884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of microRNA-204 (miR-204) on the biological characteristics of breast cancer cells.Methods Real-time PCR was used to detect the expression of miR-204 in human breast cancer cell MDA-MB-231 after transfection of miR-204 mimics and inhibitor for 48 h.Flow cytometry was used to analyse the effect of miR-204 on the proliferation and apoptosis of MDA-MB-231 cells.The effect of miR-204 on the migration of MDA-MB-231 cells was detected by Transwell migration assay.Results Real-time PCR analysis showed that miR-204 mimics and inhibitors had significant effect compared with normal control group(P<0.01).Flow cytometry analysis showed that compared with normal control group,the number of G1 phase cells of miR-204 mimics group was significantly decreased(P<0.01),while the number of G2/M cells of miR-204 mimics group was significantly increased(P<0.01).In contrast,the number of G1 phase cells of miR-204 inhibitor group was significantly increased(P<0.01),while the number of G2/M cells of miR-204 inhibitor group was significantly decreased(P<0.01).miR-204 mimics group significantly promoted apoptosis,while the inhibitor group significantly inhibited apoptosis(P<0.01).Transwell migration analysis showed that the number of cells of miR-204 mimics group were significantly reduced,while the number of cells was significantly increased in the inhibitor group(P<0.01).Conclusion We find miR-204,which can promote cell apoptosis and inhibit cell proliferation and migration,is a negative factor in the breast cancer cell line MDA-MB-231.
