Correlation analysis of NF-κB signaling pathway activated by IL-18 in CD4+ T cells and the pathogenesis of PBC
10.3969/j.issn.1671-8348.2017.14.007
- VernacularTitle:CD4+T细胞中IL-18激活的NF-κB信号通路与PBC发病机制的相关性研究
- Author:
Erchuan ZHAO
;
Hongmei LI
;
Zhijing REN
;
Yuqing HE
;
Mingzhu WANG
;
Zhenxuan YE
;
Wenjing ZHOU
;
Hua ZHANG
- Keywords:
liver cirrhosis;
biliary;
T-lymphocytes;
antigens;
CD4;
interleukin-18;
primary biliary cirrhosis;
nuclear factor-kappa signaling pathway
- From:
Chongqing Medicine
2017;46(14):1892-1896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between NF-κB signaling pathways activated by IL-18 in CD4+ T cells and the pathogenesis of PBC.Methods We detected the expression of IL-18 mRNA in PBMCs,IL-18 level in plasma,receptor IL-18R on surface of CD4+ T cell,proliferation rate of CD4+T cell and its NF-κB signaling pathway protein IκBα and NF-κB p65 by qRT-PCR,ELISA,flow cytometry,MACS and Western blot on 32 cases of patients with PBC (PBC group) and 32 healthy people (control group) in Guizhou provincial people′s hospital.Results The level of IL-18 in PBC group was significantly higher than that in control group (P<0.05).The relative expression of IL-18 mRNA in PBC group was significantly higher than that in control group (P<0.05).The percentage of CD4+T cells expressing IL-18Rα in PBC group was higher than that in control group (P<0.05).The proliferation rate of CD4+T cells stimulated by IL-18 in PBC group was significantly higher than that in healthy control group (P<0.01).The relative expression levels of NF-κB p65 protein were up-regulated in IL-18,and the expression of IκBα protein in each group was significantly increased,especially in PBC group (P<0.01).Conclusion IL-18 can activate NF-κB signal pathway in CD4+ T cells and participate in the pathogenesis of primary biliary cirrhosis.
