An improved method for isolation of human atrial cardiomyocytes

VernacularTitle:人心房肌细胞分离方法的改良研究
Author: Xiaoyu WANG ; Zhongcai FAN ; Wei ZHOU ; Yiyan YU ; Tao ZHOU ; Miaoling LI
Keywords: electricity; myocytes; cardiac; human atria myocytes; isolated method; patch-clamp technique; ionic channel
From: Chongqing Medicine 2017;46(14):1941-1943,1946
CountryChina
Language:Chinese
Abstract: Objective To study an improved isolated method of single human atrial myocytes.Methods Enzyme digestion method was used to isolate single myocytes from human atrial and whole-cell patch clamp technique was used to record small conductance calcium activated potassium current.Results This method obtained a large number of atrial myocytes.The total amount of atrial myocytes in SR group was 320±30 while AF group was 230±20 and the difference was statistically significant(P<0.01).In this study,a large number of simple and striated single atrial myocytes were obtained,and a typical small-conductance calcium-activated potassium channel current was recorded on the isolated atrial myocytes.Conclusion The established isolated method is simple,stable and effective.We can acquire a large amount of single atrial myocytes with good quality.