Analysis of amplification and bioinformatics on mycobacterium tuberculosis protein higA
10.3969/j.issn.1671-8348.2017.14.024
- VernacularTitle:结核分枝杆菌higA基因的扩增及生物信息学分析
- Author:
Na DONG
;
Dan LIU
;
Yurong FU
;
Zhengjun YI
- Keywords:
mycobacterium tuberculosis;
gene amplification;
higA;
bioinformatics analysis
- From:
Chongqing Medicine
2017;46(14):1944-1946
- CountryChina
- Language:Chinese
-
Abstract:
Objective To amplify the higA gene from the Mycobacterium tuberculosis,and to analyze the structure and function of their encoded proteins by using bioinformatics.Methods Total DNA was extracted from Mycobacterium tuberculosis.PCR of higA was performed and the products were sequenced.The biological features of the higA protein including,its physical and chemical properties,signal peptide,spatial structure and epitopes were analyzed by using software online.Results The PCR products of higA were 450 bp in length,which were consistent with the expected size.The higA protein consisted of 149 amino acids and had the following characteristics:a theoretical isoelectric point of 7.93,a fat-soluble factor of 94.30,and instability coefficient of 36.57.The higA protein had no signal peptide,containing 10 phosphorylation sites and multiple potential epitopes.Conclusion Mycobacterium tuberculosis higA gene can be amplified by PCR and the characteristics of higA protein is identified.
