Effects of Green Tea Polyphenols on LDLR Function and Potential Mechanism in HepG2 Cells
- VernacularTitle:绿茶多酚对人肝癌细胞低密度脂蛋白受体功能的影响及机制
- Author:
Feifei LI
;
Zhanpeng WEN
;
Yue GUO
;
Xiaodong ZHUANG
;
Zhimin DU
- Keywords:
epigallocatechin gallate;
coronary heart disease;
low density lipoprotein receptor;
HepG2 cells
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(3):359-363
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the effects of the bioactive constituents of green tea polyphenols epigallocatechin gallate (EGCG) on low density lipoprotein receptor (LDLR) function of HepG2 cells.[Methods] The optimal concentration and cell proliferation of HepG2 cells were determined by CCK8 assay,and Western blotting was used to determine LDLR and PCSK9 protein levels,respectively,and LDL uptake in HepG2 cells was detected by fluorescence microscope.[Results] EGCG elevated LDLR protein expression,reduced PCSK9 protein expression and promoted LDL uptake in HepG2 cells.[Conclusion] EGCG may increase LDLR abundance by down-regulating PCSK9 protein and attenuating LDLR protein degradation,which providing a new approach for lipid lowering therapy.
