Effect of Shenqi Fuzheng injection combined with IFN-α on the expression of STAT1 gene in hepatocellular carcinoma cells
10.3969/j.issn.1009-9905.2017.06.001
- VernacularTitle:参芪扶正注射液联合IFN-α对肝癌细胞STAT1基因表达的影响
- Author:
Xiaoheng CHEN
;
Bei HE
;
Huilong LI
;
Rui HU
;
Lu LI
;
Xin WANG
;
Tangshun WANG
;
Naiqing LI
;
Zhiguo DING
- Keywords:
Hepatocellular carcinoma;
MHCC97-L;
STAT1;
Interferon alpha;
Shenqifuzheng injection
- From:
Chinese Journal of Current Advances in General Surgery
2017;20(6):421-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of Shenqi Fuzheng injection combined with interferon-α (IFN-α) on the expression of STAT1 gene in hepatocellular carcinoma cells,and to elucidate its mechanism of IFN-α synergistic effect.Methords:The effect of IFN-α injection alone or combined with Shenqi Fuzheng injection on the proliferation of MHCC97-L cells was detected by MTT assay.IFN-α was detected by Real-time PCR and Western-blot respectively.The expression of STAT1 mRNA and protein in the experimental group,the negative control group (NaCl) and the blank control group were determined,and the effect of Shenqi Fuzheng injection on the transcription and expression of STAT1 was determined.The expression of STAT1 in lentiviral vector MTT assay was used to detect the effect of IFN-α alone or in combination with Shenqi Fuzheng injection on the STAT1 gene silencing cells.The expression of STAT1 was detected by RT-PCR and Western-Blot in MHCC97L cells.Strain,on cell proliferation.Results:Compared with IFN-α alone,Shenqi Fuzheng injection combined with IFN-α enhanced the inhibitory effect of IFN-α on MHCC97-L (P＜0.01) and up-regulated the expression of STAT1mRNA and protein (P＜0.05).Successfully constructed the STAT1 gene silent in the MHCC97-L cell line.There was no significant difference in the inhibition rate of MHCC97-L between Shenqi Fuzheng injection and IFN-α (P＞0.05).Conclusion Shenqi Fuzheng Injection can up-regulate the expression of STAT1 in human hepatocellular carcinoma cell line MHCC97-L,so as to increase the effect of IFN-α.
