Content Determination of Paeonol in Cynanchum paniculatum by Three-dimensional Fluorescence Coupled with ATLD Algorithm
10.6039/j.issn.1001-0408.2017.15.21
- VernacularTitle:三维荧光光谱结合交替三线性分解算法测定徐长卿药材中丹皮酚的含量
- Author:
Xuemei BAI
;
Delong LIU
;
Yongju WEI
- Keywords:
Three-dimensional fluorescence;
ATLD;
Cynanchum paniculatum;
Paeonol;
HPLC
- From:
China Pharmacy
2017;28(15):2089-2092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of paeonol in Cynanchum paniculatum by three-dimensional fluorescence coupled with ATLD algorithm. METHODS:The trilinear model was established. The principle of least square was used for ATLD. The component value was fitted by core consistency diagnosis,and inner filter effect was corrected by mathematical correction method. Fluorescence scanning condition included excitation wavelength of 250-400 nm,emission wavelength of 410-600 nm, wavelength interval of 5 nm,slit width of 5.0/5.0 nm,scanning speed of 1200 nm/min. Determination and absorption spectrum condition included scanning wavelength of 250-600 nm,scanning speed of 600 nm/min;Al(Ⅲ)was used as the sensitizer to in-crease the fluorescence intensity of paeonol. The content of paeonol was determined by HPLC. RESULTS:The linear range of pae-onol was 0.132-1.188 μg/mL(r=0.9999). RSDs of precision,stability and reproducibility tests were less than 3.0%. The recover-ies were 100.1%-104.7%(RSD=2.39%,n=6). The analysis spectrum of paeonol was almost completely overlapped with the actu-al spectrum. The results of HPLC method are very similar to those of ATLD algorithm. CONCLUSIONS:The three-dimensional fluorescence coupled with ATLD algorithm is simple,rapid,efficient and accurate,and can be used for the qualitative and quantita-tive analysis of complex system.
