Effects of PKC activation on expression of Nrf2 in rabbit RPE cells
10.13389/j.cnki.rao.2017.0106
- VernacularTitle:PKC活化对兔视网膜色素上皮(RPE)细胞Nrf2表达的影响
- Author:
Dong WEI
;
Yingying JIAO
;
Lulu QIU
;
Zhaoyi SUN
- Keywords:
protein kinase C;
phorbol 12-myristate 13-acetate;
nuclear factor enthroid 2-related factor 2;
nuclear translocation
- From:
Recent Advances in Ophthalmology
2017;37(5):419-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether activation of protein kmase C (PKC) can induce the activation and nuclear translocation of nuclear factor enthroid 2-related factor 2 (Nrf2) in retinal pigment epithelial (RPE) cells in vitro,and explore whether PKC activation may affect the expression of Nrf2 in RPE cells.Methods PKC-specific activator phorbol ester PMA was used to culture rabbit RPE cells and RPE cells pretreated with Nrf2 inhibitor for 24 hours.Immunofluorescence and Western blot were used to detect Nrf2 in the nucleus of the expression of the situation,the data were obtained for statistical analysis.Results The expression of Nrf2 protein in the nucleus of PRE cells was detected by immunofluorescence.Compared with the control group,the expression of Nrf2 protein in the nucleus of RPE cells increased in the experimental group,and the increase of PMA + Nrf2 inhibitor group was lower than that of PMA group.The difference between the two groups was statistically significant (P ＜0.05).Western blot analysis showed that the Nrf2 protein in the nucleus of PRE was quantitatively analyzed by image analysis.The gray value of the control group was significantly different (0.286 ± 0.013 in the control group,1.304 ± 0.033 in the PMA group and 0.671 ± 0.087 in the PMA + Nrf2 inhibitor group,P ＜ 0.05).Conclusion PKC can activate nuclear translocation of Nrf2 in rabbit RPE cytoplasm,and Nrf2 inhibitor can attenuate the effect of PKC.
