Simultaneous determination of four constituents in Qingzhiyi Tablets by HPLC
10.3969/j.issn.1001-1528.2017.05.017
- VernacularTitle:HPLC法同时测定情之怡片中4种成分
- Author:
Haitao ZHANG
;
Juan FU
;
Manman LI
;
Weirong BAI
;
Zhenzhong WANG
;
Wei XIAO
- Keywords:
Qingzhiyi Tablets;
gallic acid;
puerarin;
salvianolic acid B;
tanshinone ⅡA;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(5):968-971
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Qingzhiyi Tablets (Puerariae lobatae Radix,Phyllanthi Fructus,Salviae miltiorrhizae Radix et Rhizoma,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 30 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of 0.1％ formic acid-methanol-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 270 nm.RESULTS Gallic acid,puerarin,salvianolic acid B and tanshinone Ⅱ A showed good linear relationships within the ranges of 11.95-382.48,14.23-455.28,10.77-344.68 and 3.89-124.32 μg/mL,whose average recoveries were 99.96％,100.92％,98.87％ and 97.67％ with the RSDs of 1.09％,1.30％,1.11％ and 1.22％,respectively.CONCLUSION This sensitive,simple and accurate method can be used for the quality control of Qingzhiyi Tablets.
