A contrastive research on bFGF expression in retinas of rd mouse and C57BL mouse
10.13389/j.cnki.rao.2017.0104
- VernacularTitle:碱性成纤维细胞生长因子(bFGF)在遗传性视网膜变性小鼠视网膜内的表达
- Author:
Hao ZHENG
;
Genlin LI
- Keywords:
basic fibroblast growth factor;
retinal degeneration;
ganglion cell
- From:
Recent Advances in Ophthalmology
2017;37(5):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expressions of basic fibroblast growth factor (bFGF) in retinas of rd mouse (the experiments) and C57BL mouse (the controis) of five different postnatal ages,analyze the similarities and differences of bFGF expressions,and conclude the role of bFGF during the key period that retinas developed or degenerated,Methods Eight rd mice and eight C57BL mice of postnatal 0 week,1 week,2 weeks,3 weeks,4 weeks (PN 0,1,2,3,4 W) were chosen stochastically and executed immediately,their eyeballs were excavated and made into the retinal slices,then the bFGF contents in the retinas were analyzed by immunohistochemical method,photomicroscope and semi-quantitative analysis.Results Change characteristic of the curve of bFGF contents in retina of rd mice was similar to that of C57BL mice.Both curves began to fall after born,which reached each low level at PN-1W and then rose until reaching each peak at PN-2W,at which ten layer structures of retinas in both groups were formed.Then both curves began the second fall till PN-4W.During the last two weeks,retinas of C57BL mice degenerated rapidly while retinas of rd mice continued to mature.For all the five postnatal weeks,the bFGF contents in retinas of rd mice were lower than those of C57BL mice evidently.All statistical analysis of the difference have made sense distinctly (P ＜ 0.01).Couclusion bFGF may play a protective role in the developing to maturation and sustaining of retinal structure,and the higher expression of bFGF content may prevent retinal degeneration in some extent.
