Simultaneous determination of six constituents in Qingre Zhili Pills by HPLC
10.3969/j.issn.1001-1528.2017.05.014
- VernacularTitle:HPLC法同时测定清热治痢丸中6种成分
- Author:
Xi ZHAO
;
Dongsheng YANG
;
Yao ZHANG
;
Zhongbao LI
;
Xiaodong ZHOU
- Keywords:
Qingre Zhili Pills;
berberine hydrochloride;
jatrorrhizine hydrochloride;
palmatine hydrochloride;
kaempferol;
luteolin;
apigenin;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(5):956-959
- CountryChina
- Language:Chinese
-
Abstract:
AIM To develop an HPLC method for the simultaneous content determination of berberine hydrochloride,jatrorrhizine hydrochloride,palmatine hydrochloride,kaempferol,luteolin and apigenin in Qingre Zhili Pills (Berberidis Radix and Portulacae Herba).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.02％ potassium dihydrogen phosphate flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 265 nm and 360 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r≥0.999 3),whose average recoveries were 97.16％-99.90％ with the RSDs of 0.62％-1.48％.CONCLUSION This simple method can be used for the rapid quality control of Qingre Zhili Pills.
