Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination: A Preliminary Study.
- Author:
Ji Hyae LIM
1
;
So Yeon PARK
;
Shin Young KIM
;
Do JinDo KIM
;
Mee Jin KIM
;
Jae Hyug YANG
;
Moon Young KIM
;
Min Hyoung KIM
;
Ho Won HAN
;
Kyu Hong CHOI
;
Hyun Mee RYU
Author Information
1. Laboratory of Medical Genetics, Medical Research Institute, Cheil General Hospital and Women's Healthcare Center, Seoul, Korea. hmryu@yahoo.com
- Publication Type:Original Article
- Keywords:
Cell free DNA (cf-DNA);
Quantitative fluorescent-polymerase chain reaction (QF-PCR);
Non-invasive fetal gender detection
- MeSH:
Cohort Studies;
DNA;
DNA, Viral;
Female;
Genes, sry;
Humans;
Plasma;
Pregnancy;
Prospective Studies
- From:Journal of Genetic Medicine
2010;7(1):53-58
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system (viral kit) for non-invasive first-trimester fetal gender determination. MATERIALS AND METHODS: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. RESULTS: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. CONCLUSION: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.
