Effects of Chrysophanol on Learning and Memory Impairment Induced by Sesflurane in Aged Rats
10.3870/j.issn.1004-0781.2017.04.007
- VernacularTitle:大黄酚对地氟醚诱导老年大鼠学习认知功能损伤的影响
- Author:
Zhan LIU
- Keywords:
Chrysophanol;
Desfluran;
Aβ1-42 peptide;
Apoptosis;
Learning abilities;
Memory abilities
- From:
Herald of Medicine
2017;36(4):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of inhalation anesthestic desfluran (Des) on the learning and memory abilities of rats and the protective role of chrysophanol.Methods Totally,50 male and 50 female rats,aging 24 months and weighing (500 ± 10) g,were randomly divided into five groups:normal control group,Des group,low-,mediumand high-dose Chr group (0.1,1.0 and 10.0 mg·kg-1),with 20 rats in each group.After anesthetization for 24 h,the Morris water maze was used to investigate the abilities of learning and memory of rats.The amount of Aβ1-42 was determined by ELISA assay,and the apoptosis of rat hippocampal neurons in five group was observed by TUNEL assay.Furthermore,the expression levels of Bcl-2,Bax and Caspase-3 were examined by Western blotting.The activity of acetylcholinesterase in each rats hippocampus was determined using iron trichloride chromogenic spectrophotometer colorimetric analysis method.Results Compared with the normal control group,the mean escape latency of the rats in Des group was significantly prolonged;the spatial exploring time (29.85 ± 4.51) s was reduced;the apoptotic rate of neurons (0.742 ± 0.052)％,the amount of Aβ1-42 peptide (9 618.72 ± 1 076.43) pmol· g-1,the expression levels of Caspase-3 (1.132 ± 0.217),and Bax (1.298 ± 0.209) were increased;the expression of Bcl-2 (0.318 ±0.038) were reduced;the activity of acetylcholinesterase (96.38 ±7.62) U·mL-1 was increased.Compared with the Des group,the rats in all Chr groups obtained shorter escape latency and longer spatial exploring time;the amount of Aβ1-42 peptide and the expression levels of Caspase-3 and Bax were down-regulated;the activity of acetylcholinesterase was reduced.In addition,chrysophanol improved the abilities of learning and memory of anesthetic rats in a dose-dependent manner.Conclusion Chrysophanol could improve the abilities of the learning and memory of rats after desflurane anesthesia,along with inhibition of Aβ deposition.
