Enrichment of Total Flavonoids from Seed Shell of Scutellaria Baicalensis by Macroporous Resin
10.3870/j.issn.1004-0781.2017.04.016
- VernacularTitle:大孔树脂法富集黄芩籽种壳总黄酮
- Author:
Li YANG
;
Hongwei WANG
;
Hongbo LI
;
Guiqin ZHAO
;
Xiaofeng ZHANG
- Keywords:
Scutellarin;
Total Favonoids;
Seed shell;
Orthogonal experiment test;
Macroporous resin
- From:
Herald of Medicine
2017;36(4):420-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize enrichment and purification technology of total flavonoids from seed shell of Scutellaria baicalensis by macroporous resin.Methods Scutellarin was selected as the index,and its content was determined by HPLC.Types of macroporous resin were optimized by static adsorption elution tests.Enrichment and purification technology was investigated by orthogonal test and single-factor tests.Results AB-8 macroporous resin showed better effects than others.Optimum purification condition was as follows:sample concentration was 30.0 g · L-1,the best adsorption capacity was 0.3 g· g-1,adsorption velocity was 1.0 mL· min-1,diameter-height ratio was 1 ∶ 3,the eluting agent was 20 BV purified water,with the total polyphenols being desorbed by 5 BV of 70％ ethanol Conclusion The technology is scientific,simple and highly reproducible.It can be applied to enrich and purify total flavonoids from seed shell of Scutellaria baicalensis by macroporous resin.
