Determination of Contents of Five Indole Alkaloids in Rauvolfia by High Performance Liquid Chromatography
10.3870/j.issn.1004-0781.2017.04.017
- VernacularTitle:高效液相色谱法测定萝芙木中5种吲哚类生物碱的含量
- Author:
Wenjing LI
;
Jin YE
;
Dezhu YANG
;
Li LI
;
Bo HONG
;
Jie ZHANG
;
Xin LIANG
;
Tingting LIU
- Keywords:
Rauvolfia;
Indole alkaloids;
Content determination;
Quality control;
Chromatography,high performance liquid
- From:
Herald of Medicine
2017;36(4):425-428
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an high perfermance liquid chromatography method for determination of five indole alkaloids in Rauvolfia.Methods The Diamonsil C18 column was used at 25 ℃ with methanol-water (gradient elution) as the mobile phase,the flow rate was 1.0 mL·min-1,the detective wavelength was 280 nm,and the injection volume was 5 μL.Results In the range of 1.56-200 μg· mL-1,the correlation coefficients of regression equations of sarpagine,yohimbine,ajmaline,ajmalicine,reserpine were higher than 0.999 0.The average recovery (n =9) of five indole alkaloids was between 95.0％-105.0％.Conclusion This method is simple with good accuracy and repeatability.It can be used for quality control of Rauvolfia.
